Scientific Overview

Three areas related to HIV-1 transmission and pathogenesis are under study:

• Pathogenesis and transmission of X4 and R5 SIV/HIV (SHIV) chimeric viruses
• Genotypic and phenotypic determinants of SHIV pathogenesis
• Efficacy of candidate topical microbicides in prevention of HIV-1 transmission.

Our laboratory focuses on the use of non-human primate (NHP) models to study HIV infection and the progressive development of AIDS. Experimental infections of rhesus macaques with CXCR4- and CCR5-tropic simian-human immunodeficiency viruses (SHIVs) are conducted to assess the impact of coreceptor usage in virus transmission and pathogenesis, as well as to identify the genotype and phenotype of the transmitted and pathogenic viruses. Most HIV-1 transmission results in a predominantly R5 virus infection. With time, X4 variants arise and coexist with R5 virus varainats in ~50% of subtype B infected individuals, and this event is associated with rapid CD4+ T cell loss and disease progression. We recently observed X4 virus evolution in a R5 SHIV infected macaque as disease progresses, demonstrating that coreceptor switch can happen in an animal model of HIV/AIDS. The identification of an R5 virus that can switch to CXCR4 usage in a relevant animal model of HIV/AIDS provides the experimental system critically needed to dissect the mechanistic basis for and obstacles to coreceptor switch in vivo. This system can also be employed to determine whether the use of CCR5 inhibitors will hasten the pace of X4 virus evolution.

NHP studies are also an important tool for delineating the correlates of protection, information of which will contribute to the design of improved AIDS vaccine candidates. For these reasons, we are actively engaged in characterizing the breath, depth and site of immune responses in macaques that control viremia to levels below the limit of detection, and in those that are continuously exposed and yet remain negative for serum antibodies and detectable virus. Lastly, given the current epidemic trend, there is an urgent need to expand the range of interventions such as the use of topical microbicides that can be controlled by women. Experiments are being conducted to assess the safety, distribution and efficacy of candidate topical microbicides in the SHIV/macaque model. For the latter purpose, a repeated low-dose mucosal exposure protocol that more likely mimics HIV-1 sexual transmission in humans has been established. Using this challenge protocol, synergism between suboptimal vaccines and topical microbicides in conferring protection against virus transmission will also be evaluated.

The main research efforts of the Cheng-Mayer laboratory are aimed at advancing our understanding of the mechanisms of HIV transmission and pathogenesis. Infection of non-human primates (NHP) with simian immunodeficiency viruses carrying the envelope glycoproteins of HIV-1 reproduces many features of HIV-1 infection in humans. Using this animal model, we sort to determine whether certain virus variants are more transmissible or are better at causing disease in the hosts. The ways in which the virus evolves from a less pathogenic towards more pathogenic variants, as well as the specific immune responses (antibodies, CD4+ or CD8+ T cell responses, or combination of these) that protect macaques from infection or disease development are also being studied. The latter will provide vital clues to the type of responses that would be required to protect against HIV infection in humans. Lastly, the increasing feminization of the AIDS pandemic brings into focus the need to expand the range of interventions, such as topical microbicides that can be controlled by women. We are actively engaged, using the macaque model, in identifying safe and efficacious microbicide products that could be advanced for clinical testing in humans.

NAME: Cecilia Cheng-Mayer

POSITION TITLE: Professor/Staff Investigator

EDUCATION

1972	A.B. (Bacteriology)   University of California, Berkeley
1978	Ph.D (Microbiology)   Columbia University, New York
1981	Post-Doc (Developmental Genetics)   Sloan-Kettering Institute for Cancer Research

PROFESSIONAL EXPERIENCE

1973-1974	Teaching Assistant in Medical Microbiology, Columbia University, NY
1975	Teaching Assistant in Infectious Disease, Columbia University, NY
1981-1982	Associate Researcher, Sloan-Kettering Institute for Cancer Research
1982-1985	Research Assistant, University of Miami School of Medicine, Miami
1985-1986	Research Scholar, University of California, San Francisco
1986-1989	Assistant Research Virologist, Cancer Research Institute UCSF School of Med.
1990-1994	Associate Research Virologist, Cancer Research Institute UCSF School of Med.
1994-Present	Staff Investigator, The Aaron Diamond AIDS Research Center, NY
1994-2002	Associate Professor, The Rockefeller University, NY
2003-	Professor, The Rockefeller University, NY

PUBLICATIONS (SELECTED)

• Cheng-Mayer C, Seto D, Tateno M and Levy JA. 1988. Biologic features of HIV that correlate with virulence in the host. Science 240: 80-82.
• Shioda T, Levy JA and Cheng-Mayer C. 1991. Macrophage and T cell line tropisms of HIV 1 are determined by specific regions of the envelope gp120. Nature 349: 167-169.
• Shioda T, Levy JA, and Cheng-Mayer C. 1992. Small amino acid changes in the V3 hypervariable region of gp120 can affect the T-cell and macrophage tropism of human immunodeficiency virus type 1. Proc Natl Acad Sci USA, 89:9434-9438.
• Stamatatos L and Cheng-Mayer C. 1993. Evidence that the structural conformation of envelope gp120 affects HIV-1 infectivity, host range and syncytium-forming ability. J Virol. 67:5635-5639.
• Koito A, Harrowe G, Levy JA and Cheng-Mayer C. 1994. Functional role of the V1/V2 domain of human immunodeficiency virus type 1 envelope glycoprotein gp120 in infection of primary macrophages and soluble CD4 neutralization. J Virol, 68:2253-2259.
• Allan JS, Ray R, Broussard S, Whitehead E, Hubbard G, Butler T, Brasky K, Luciw P, Cheng-Mayer C, Levy JA, Steimer K, Li J, Sodroski J and Garcia-Moll, M. 1995. Infection of baboons with simian/human immunodeficiency viruses. JAIDS 9:429-441.
• Luciw PA, Pratt-Lowe E, Shaw KES, Levy JA and Cheng-Mayer C. 1995. Persistent infection of rhesus macaques with T-cell line-tropic and macrophage-tropic clones of simian/human immunodeficiency viruses (SHIV). Proc Natl Acad Sci 92:7490-7494.
• Stamatatos L and Cheng-Mayer C. 1995. Structural modulations of the envelope gp120 glycoprotein of HIV-1 upon oligomerization and differential V3 loop-epitope exposure for isolates displaying distinct tropism upon virion-soluble receptor binding. J Virol, 69:6191-6198.
• Trkola A, Dragic T, Arthos J, Binley JM, Olson WC, Allaway GP, Cheng-Mayer C, Robinson J, Maddon PJ and Moore JP 1996. Interference with HIV-1 gp120 binding to the chemokine receptor CCR-5 by neutralizing antibodies. Nature 384:184-187.
• Cheng-Mayer C, Liu R, Landau NR and Stamatatos L. 1997. Macrophage tropism of HIV-1 and utilization of the CC-CKR5 coreceptor. J Virol, 71:1657-1661.
• Harouse JM, Tan RCH, Gettie A, Dailey P, Marx PA, Luciw PA and Cheng-Mayer C. 1998. Mucosal transmission of pathogenic CXCR4-utilizing SHIVSF33A variants in rhesus macaques. Virol, 248:95-107.
• Stamatatos L and Cheng-Mayer C. 1998. An envelope modification that renders a primary, neutralization resistant, clade B HIV-1 isolate highly susceptible to neutralization by sera from other clades. J Virol, 72:7840-7845.
• Luciw PA, Mandell C, Himathongkham S, Li J, Low TA, Schmidt KA, Shaw KES and Cheng-Mayer C. 1999. Fatal immunopathogenesis by SIV/HIV-1 (SHIV) containing the HIV-1SF33 env gene in juvenile and newborn rhesus macaques. Virol, 263:112-127.
• Cheng-Mayer C, Brown A, Harouse J, Luciw PA and Mayer AJ. 1999. Selection for neutralization resistance of the SHIVSF33A variant in vivo by virtue of sequence changes in the extracellular envelope glycoprotein that modify N-linked glycosylation. J Virol, 73:5294-5300.
• Harouse JM, Gettie A, Tan RCH, Blanchard J and Cheng-Mayer C. 1999. Distinct pathogenic sequela in rhesus macaques infected with CCR5 or CXCR4 utilizing SHIVs. Science, 284:816-819.
• Brown A, Wang X, Sawai E and Cheng-Mayer C. 1999. Activation of the PAK-related kinase by HIV-1 Nef in primary human peripheral blood lymphocytes and macrophages leads to phosphorylation of a PIX/p95 complex. J Virol, 73:9899-9907.
• Chakrabarti LA, Lewin SR, Zhang L, Gettie A, Luckay A, Martin LN, Skulsky E, Ho DD,Cheng-Mayer C, Marx PA 2000. Normal T-cell turnover in Sooty Mangabeys harboring active simian immunodeficiency virus infection. J Virol, 74: 1209-1223.
• Chakrabarti L, Lewin SR, Zhang L, Gettie A, Luckay A, Martin LN, Skulsky E, Ho DD, Cheng-Mayer C and Marx PA. 2000. Age-dependent changes in T cell homeostasis and SIV load in sooty mangabeys. J Med Primatol, 29:158-165.
• Harouse JM, Gettie A, Eshetu T, Tan RCH, Bohm R, Blanchard J, Baskin G and Cheng-Mayer C. 2001. Mucosal transmission and induction of simian AIDS by CCR5-specific SHIV SF162P. J Virol, 75:1990-1995.
• Malenbaum S, Yang D and Cheng-Mayer C. 2001. Evidence for similar recognition of the conserved neutralization epitopes of HIV-1 gp120 in humans and macaques. J Virol, 75:9287-9296.
• Parren PWHI, Marx PA, Hessell AJ, Luckay A, Harouse J, Cheng-Mayer C, Moore JP and Burton DR 2001. Antibody protects against vaginal challenge with a pathogenic R5 simian/human immunodeficiency virus at serum levels giving complete neutralization in vitro. J Virol, 75:8340-8347.
• Chakrabarti L, Ivanovic T and Cheng-Mayer C. 2002. Properties of the surface envelope glycoprotein associated with virulence of Simian-Human Immunodeficiency virus SHIVSF33A molecular clones. J Virol, 76:1588-1599.
• Lue,J, Hsu M, Yang D, Marx P, Chen Z and Cheng-Mayer C. 2002. Addition of a single gp120 glycan confers increased binding to DC-SIGN and neutralization escape to HIV-1. J Virol, 76:10299-10306.
• Hsu M, Harouse JM, Gettie A, Buckner C, Blanchard J and Cheng-Mayer C. 2003. Increased mucosal transmission but not enhanced pathogenicity of the CCR5-tropic, SAIDS-inducing SHIVSF162P3 maps to envelope gp120. J Virol, 77:989-998.
• Chakrabarti LA, Metzner KJ, Ivanovic T, Cheng H, Louis-Virelizier J, Connor RI and Cheng-Mayer C. 2003. A multiply deleted form of Nef selected during pathogenic reversion of SIVmac239-Δnef increases viral replication. J Virol, 77:1588-1599.
• Hsu M, Zhang J, Flint M, Logvinoff C, Cheng-Mayer C, Rice CM and McKeating J. 2003. Hepatitis C virus glycoproteins mediate pH-dependent fusion and cell entry of pseudotyped retroviral particles. Proc Natl Acad Sci USA, 100:7271-7276.
• Harouse JM, Buckner C, Gettie A, Fuller R, Bohm R, Blanchard J and Cheng-Mayer C. 2003. CD8+ T cell-mediated CXCR4-SHIV suppression in dually-infected rhesus macaques. Proc Natl Acad Sci USA, 100:10977-10982.
• Reyes RA, Canfield DR, Esser U, Adamson LA, Cheng-Mayer C, Gardner M, Harouse JM and Luciw PA. 2004. Induction of simian AIDS in neonatal rhesus macaques infected with CCR5 and CXCR4 utilizing SHIV is associated with distinct lesions of the thymus. J Virol, 78:2121-2130.
• Balfe P, Shapiro S, Hsu M, Buckner C, Harouse J and Cheng-Mayer C. 2004. Expansion of quasispecies diversity but no evidence for adaptive evolution of SHIV during rapid serial transfers between seronegative macaques. Virol, 318:267- 279.
• Brown A, Moghaddam S, Kawano T and Cheng-Mayer C. 2004. Multiple human immunodeficiency virus type 1 Nef functions contribute to efficient replication in primary human macrophages. J Gen Virol, 85:1463-1469.
• Saunders CJ, McCaffrey RA, Zharkikh I, Kraft Z, Malenbaum SE, Burke B, Cheng-Mayer C and Stamatatos L. 2005. The V1, V2, and V3 regions of the human immunodeficiency virus type 1 envelope differentially affect the viral phenotype in an isolate-dependent manner. J Virol, 79:9069-9080.
• Cheng H, Cenciarelli C, Nelkin G, Tsan R, Fan C, Cheng-Mayer C and Fidler IJ. 2005. Molecular mechanism of hTid-1, the human homolog of Drosophila tumor suppressor l(2)Tid, in the regulation of NF-kappaB activity and suppression of tumor growth. Mol Cell Biol, 25:44-59.
• Brown A, Gartner S, Kawano T, Benoit N and Cheng-Mayer C. 2005. HLA-A2 down-regulation on primary human macrophages infected with an M-tropic EGFP-tagged HIV-1 reporter virus. J Leukoc Biol, 78:675-685.
• Ratterree M, Gettie A, Williams V, Malenbaum S, Neurath AR, Cheng-Mayer C and Blanchard J. 2005. Safety and distribution of cellulose acetate 1,2-benzenedicarboxylate (CAP), a candidate anti-HIV microbicide in rhesus macaques. AIDS, 19:1595-1599.
• Hsu M, Ho SH, Balfe P, Gettie A, Harouse J, Blanchard J and Cheng-Mayer C. 2005. A CCR5-tropic simian-HIV molecular clone capable of inducing AIDS in rhesus macaques. J Acquir Immune Defic Syndr 40:383-387.
• Ho SH, Shek L, Gettie A, Blanchard J and Cheng-Mayer C. 2005. V3 loop-determined coreceptor preference dictates the dynamics of CD4+-T-cell loss in simian-human immunodeficiency virus-infected macaques. J Virol, 79:12296-12303.
• Boadi T, Schneider E, Chung S, Tsai L, Gettie A, Ratterree M, Blanchard J, Neurath AR and Cheng-Mayer C. 2005. Cellulose acetate 1,2-benzenedicarboxylate protects against challenge with pathogenic X4 and R5 simian/human immunodeficiency virus. AIDS 19:1587-1594.
• Trunova N, Tsai L, Tung S, Schneider E, Harouse J, Gettie A, Simon V, Blanchard J and Cheng-Mayer C. 2006. Progestin-based contraceptive suppresses cellular immune responses in SHIV-infected rhesus macaques. Virology, 352:169-177.
• Ho SH, Martin F, Higginbottom A, Partridge LJ, Parthasarathy V, Moseley GW, Lopez P, Cheng-Mayer C and Monk PN. 2006. Recombinant extracellular domains of tetraspanin proteins are potent inhibitors of the infection of macrophages by human immunodeficiency virus type 1. J Virol 80:6487-96.
• Tsai L, Trunova N, Gettie A, Mohri H, Bohm R, Saifuddin M, Cheng-Mayer C. 2007. Efficient repeated low-dose intravaginal infection with X4 and R5 SHIVs in rhesus macaque: Implications for HIV-1 transmissions in humans. Virology, 362:207-216.
• Tasca S, Tsai L, Trunova N, Gettie A, Saifuddin M, Bohm R, Chakrabarti L, Cheng-Mayer C. 2007. Induction of potent local cellular immunity with low dose X4 SHIVSF33A vaginal exposure: correlations with protection from re-exposure. Virol. 367:196-211
• Ho SH, Tasca S, Shek L, Li A, Gettie A, Blanchard J, Boden D, Cheng-Mayer C. 2007. Coreceptor switch in a macaque infected with CCR5 (R5)-tropic simian-human immunodeficiency virus. J. Virol. 81:8621-8633.
• Tasca S, Tsai L, Trunova N, Gettie A, Saifuddin M, Bohm R, Chakrabarti L, Cheng-Mayer C. 2007. Induction of potent local cellular immunity with low dose X4 SHIV(SF33A) vaginal exposure. Virology. 367(1):196-211
• Ho SH, Trunova N, Gettie A, Blanchard J, Cheng-Mayer C. 2008. Different mutational pathways to CXCR4 coreceptor switch of CCR5-using simian-human immunodeficiency virus. J Virol. 82(11):5653-6
• Tasca S, Ho SH, Cheng-Mayer C. 2008. R5X4 viruses are evolutionary, functional, and antigenic intermediates in the pathway of a simian-human immunodeficiency virus coreceptor switch. J Virol. 82(14):7089-99.
• Morgan C, Marthas M, Miller C, Duerr A, Cheng-Mayer C, Desrosiers R, Flores J, Haigwood N, Hu SL, Johnson RP, Lifson J, Montefiori D, Moore J, Robert-Guroff M, Robinson H, Self S, Corey L. 2008. The use of nonhuman primate models in HIV vaccine development. PLoS Med. 5(8):e173.
• Cheng-Mayer C, Tasca S, Ho SH. 2009. Coreceptor switch in infection of nonhuman primates. Curr HIV Res. 7(1):30-8.
• Wallace GS, Cheng-Mayer C, Schito ML, Fletcher P, Miller Jenkins LM, Hayashi R, Neurath AR, Appella E, Shattock RJ. 2009. HIV-1 Nucleocapsid Inhibitors Impede Trans Infection in Cellular and Explant Models and Protect Non-Human Primates from Infection. J Virol. 2009 Jul 8.
• Ren W, Tasca S, Zhuang K, Gettie A, Blanchard J, Cheng-Mayer C. 2009. Different tempo and anatomic location of dual-tropic and X4 virus emergence in a model of R5 simian-human immunodeficiency virus infection. J Virol. 2010. 84(1):340-51.
• Tasca S, Zhuang K, Gettie A, Knight H, Blanchard J, Westmoreland S, Cheng-Mayer C. Effect of B-cell depletion on coreceptor switching in R5 simian-human immunodeficiency virus infection of rhesus macaques. J Virol. 2011. 85(7):3086-94.
• Sina ST, Ren W, Cheng-Mayer C. Coreceptor use in nonhuman primate models of HIV infection. J Transl Med. 2011 Jan 27;9 Suppl 1:S7.
• Cheng-Mayer C, Huang Y, Gettie A, Tsai L, Ren W, Shakirzyanova M, Sina ST, Trunova N, Blanchard J, Jenkins LM, Lo Y, Schito ML, Appella E. Delay of simian human immunodeficiency virus infection and control of viral replication in vaccinated macaques challenged in the presence of a topical microbicide. AIDS. 2011 Sep 24;25(15):1833-1841.
• Zhuang K, Finzi A, Tasca S, Shakirzyanova M, Knight H, Westmoreland S, Sodroski J, Cheng-Mayer C. Adoption of an "open" envelope conformation facilitating CD4 binding and structural remodeling precedes coreceptor switch in R5 SHIV-infected macaques. PLoS One. 2011;6(7):e21350.
• Shakirzyanova M, Tsai L, Ren W, Gettie A, Blanchard J, Cheng-Mayer C. Pathogenic Consequences of Vaginal Infection with CCR5-Tropic Simian-Human Immunodeficiency Virus SHIVSF162P3N. J Virol. 2012;86(17):9432-42.

• Harouse, J.M., Gettie, A., Tan, R.C.H., Blanchard, J., and Cheng-Mayer, C. (1999) Distinct pathogenic sequela in rhesus macaques infected with CCR5 or CXCR4 utilizing SHIVs. Science 284:816-9. [view]
• Brown, A., Wang, X., Sawai, E. and Cheng-Mayer, C. (1999) Activation of the PAK-related kinase by HIV-1 Nef in primary human peripheral blood lymphocytes and macrophages leads to phosphorylation of a PIX/p95 complex. J. Virol. 73:9899-907. [view]
• Stamatatos, L., Lim, M. and Cheng-Mayer, C. (2000) Generation and structural analysis of soluble oligomeric envelope proteins derived from neutralization-resistant and neutralization-susceptible primary HIV-1 isolates. AIDS Res. Hum. Retro. 16: 981-94. [view]
• Malenbaum, S., Yang, D., Cavacini, L., Posner, M., Robinson, J. and Cheng-Mayer, C. (2000) The N-terminal V3 loop glycan modulates the interaction of clades A and B HIV-1 envelopes with CD4 and chemokine receptors. J. Virol. 74:11008-16. [view]
• Harouse, J.M., Gettie, A., Eshetu, T., Tan, R.C.H., Bohm, R., Blanchard, J., Baskin, G. and Cheng-Mayer, C. (2001) Mucosal transmission and induction of simian AIDS by CCR5-specific SHIVSF162P3: relevance for vaccine development. J. Virol. 75:1990-5. [view]
• Harouse JM, Gettie A, Tan RC, Eshetu T, Ratterree M, Blanchard J, Cheng-Mayer C. (2001) Pathogenic determinants of the mucosally transmissible CXCR4-specific SHIV(SF33A2) map to env region. J Acquir Immune Defic Syndr. Jul 1;27(3):222-8. [view]
• Malenbaum, S., Yang, D. and Cheng-Mayer, C. (2001) Evidence for similar recognition of the conserved neutralization epitopes of HIV-1 gp120 in humans and macaques. J. Virol., 75(19):9287-96. [view]
• Chakrabarti LA, Metzner KJ, Ivanovic T, Cheng H, Louis-Virelizier J, Connor RI, Cheng-Mayer C. A truncated form of Nef selected during pathogenic reversion of simian immunodeficiency virus SIVmac239Deltanef increases viral replication. J Virol. 2003 Jan;77(2):1245-56. [view]
• Hsu M, Zhang J, Flint M, Logvinoff C, Cheng-Mayer C, Rice CM, McKeating JA. (2003) Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles. Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7271-6. [view]
• Harouse JM, Buckner C, Gettie A, Fuller R, Bohm R, Blanchard J, Cheng-Mayer C. (2003) CD8+ T cell-mediated CXC chemokine receptor 4-simian/human immunodeficiency virus suppression in dually infected rhesus macaques. Proc Natl Acad Sci. 100(19):10977-82. [view]
• Reyes RA, Canfield DR, Esser U, Adamson LA, Brown CR, Cheng-Mayer C, Gardner MB, Harouse JM, Luciw PA. (2004) Induction of simian AIDS in infant rhesus macaques infected with CCR5- or CXCR4-utilizing simian-human immunodeficiency viruses is associated with distinct lesions of the thymus. J Virol. 78(4):2121-30. [view]
• Brown A, Moghaddam S, Kawano T, Cheng-Mayer C. (2004) Multiple human immunodeficiency virus type 1 Nef functions contribute to efficient replication in primary human macrophages. J Gen. Virol. 85(6):1463-9. [view]
• Balfe P, Shapiro S, Hsu M, Buckner C, Harouse JM, Cheng-Mayer C.. (2004) Expansion of quasispecies diversity but no evidence for adaptive evolution of SHIV during rapid serial transfers among seronegative macaques. Virology. 318(1):267-79. [view]
• Brown, A., Moghaddam, S., Kawano, T. and Cheng-Mayer, C. (2004) Multiple human immunodeficiency virus type 1 Nef functions contribute to efficient replication in primary human macrophages. J. Gen. Virol.:85: 1463-9. [view]
• Cheng, H., Cenciarelli, C., Nelkin, G., Tsan, R., Cheng-Mayer, C. and Fidler, I.J. (2005) Molecular mechanisms of hTid-1, the human homologue of Drosophila tumor suppressor (l2) Tid, in the regulation of NF-B activity and suppression of tumor growth. Mol Cell Biol 25: 44-59. [view]
• Saunders, C.J., McCaffrey, R.A., Zharkikh, I., Kraft, Z., Malenbaum, S., Burke, B., Cheng-Mayer, C. and Stamatatos, L. (2005) The V1, V2 and V3 regions of the HIV-1 envelope differentially affect the viral phenotype in an isolate-dependent manner. J. Virol. 79: 9069-80. [view]
• Brown, A., Gartner, S., Kawano, T., Benoit, N. and Cheng-Mayer, C. (2005) HLA-A2 down-regulation on primary human macrophages infected with an M-tropic EGFP-Tagged HIV-1 reporter virus. J. Leuk. Biol. 78: 675-85. [view]
• Ho, S.-H., Shek, L., Gettie, A., Blanchard, J. and Cheng-Mayer, C. (2005) V3 loop determined coreceptor preference dictates the dynamics of CD4+ T cell loss in SHIV infected macaques. J. Virol. 79: 12296-303. [view]
• Ratterree, M., Gettie, A., Williams, V., Malenbaum, S., Neurath, R.A., Cheng-Mayer, C. and Blanchard, J. (2005) Safety and distribution of cellulose acetate 1,2-benzenedicarboxylate (CAP), a candidate anti-HIV microbicide in rhesus macaques. AIDS 19: 1595-9. [view]
• Boadi, T., Schneider, E., Chung, S., Tsai, L., Gettie, A., Ratterree, M., Blanchard, J., Neurath, R.A. and Cheng-Mayer, C. (2005) Cellulose acetate 1,2-benzenedicarboxylate protects against challenge with pathogenic X4 and R5 simian-human immunodeficiency viruses. AIDS 19: 1587-94. [view]
• Hsu, M., Ho, S.-H., Balfe, P., Gettie, A., Harouse, J., Blanchard, J. and Cheng-Mayer, C. (2005) A CCR5-tropic simian-immunodeficiency virus molecular clone that is pathogenic in rhesus macaques. J Acquir Immune Defic Syndr. 2005 Dec 1;40(4):383-7. [view]
• Ho SH, Shek L, Gettie A, Blanchard J and Cheng-Mayer C. 2005. V3 loop-determined coreceptor preference dictates the dynamics of CD4+-T-cell loss in simian-human immunodeficiency virus-infected macaques. J Virol, 79:12296-303. [view]
• Boadi T, Schneider E, Chung S, Tsai L, Gettie A, Ratterree M, Blanchard J, Neurath AR and Cheng-Mayer C. 2005. Cellulose acetate 1,2-benzenedicarboxylate protects against challenge with pathogenic X4 and R5 simian/human immunodeficiency virus. AIDS 19:1587-94. [view]
• Trunova N, Tsai L, Tung S, Schneider E, Harouse J, Gettie A, Simon V, Blanchard J and Cheng-Mayer C. 2006. Progestin-based contraceptive suppresses cellular immune responses in SHIV-infected rhesus macaques. Virology, 352:169-77. [view]
• Ho SH, Martin F, Higginbottom A, Partridge LJ, Parthasarathy V, Moseley GW, Lopez P, Cheng-Mayer C and Monk PN. 2006. Recombinant extracellular domains of tetraspanin proteins are potent inhibitors of the infection of macrophages by human immunodeficiency virus type 1. J Virol 80:6487-96. [view]
• Tsai L, Trunova N, Gettie A, Mohri H, Bohm R, Saifuddin M, Cheng-Mayer C. 2007. Efficient repeated low-dose intravaginal infection with X4 and R5 SHIVs in rhesus macaque: Implications for HIV-1 transmissions in humans. Virology. 362(1):207-16. [view]
• Ho SH, Tasca S, Shek L, Li A, Gettie A, Blanchard J, Boden D, Cheng-Mayer C. 2007. Coreceptor switch in R5-tropic simian/human immunodeficiency virus-infected macaques. J Virol. 81(16):8621-33. [view]
• Tasca S, Tsai L, Trunova N, Gettie A, Saifuddin M, Bohm R, Chakrabarti L, Cheng-Mayer C. 2007. Induction of potent local cellular immunity with low dose X4 SHIV(SF33A) vaginal exposure. Virology. 367(1):196-211. [view]
• Ho SH, Trunova N, Gettie A, Blanchard J, Cheng-Mayer C. 2008. Different mutational pathways to CXCR4 coreceptor switch of CCR5-using simian-human immunodeficiency virus. J Virol. 82(11):5653-6. [view]
• Tasca S, Ho SH, Cheng-Mayer C. 2008. R5X4 viruses are evolutionary, functional, and antigenic intermediates in the pathway of a simian-human immunodeficiency virus coreceptor switch. J Virol. 82(14):7089-99. [view]
• Morgan C, Marthas M, Miller C, Duerr A, Cheng-Mayer C, Desrosiers R, Flores J, Haigwood N, Hu SL, Johnson RP, Lifson J, Montefiori D, Moore J, Robert-Guroff M, Robinson H, Self S, Corey L. 2008. The use of nonhuman primate models in HIV vaccine development. PLoS Med. 5(8):e173. [view]
• Cheng-Mayer C, Tasca S, Ho SH. 2009. Coreceptor switch in infection of nonhuman primates. Curr HIV Res. 7(1):30-8. [view]
• Wallace GS, Cheng-Mayer C, Schito ML, Fletcher P, Miller Jenkins LM, Hayashi R, Neurath AR, Appella E, Shattock RJ. 2009. HIV-1 Nucleocapsid Inhibitors Impede Trans Infection in Cellular and Explant Models and Protect Non-Human Primates from Infection. J Virol. 2009 Jul 8. [view]
• Ren W, Tasca S, Zhuang K, Gettie A, Blanchard J, Cheng-Mayer C. Different tempo and anatomic location of dual-tropic and X4 virus emergence in a model of R5 simian-human immunodeficiency virus infection. J Virol. 2010. 84(1):340-51. [view]
• Tasca S, Zhuang K, Gettie A, Knight H, Blanchard J, Westmoreland S, Cheng-Mayer C. Effect of B-cell depletion on coreceptor switching in R5 simian-human immunodeficiency virus infection of rhesus macaques. J Virol. 2011. 85(7):3086-94. [view]
• Sina ST, Ren W, Cheng-Mayer C. Coreceptor use in nonhuman primate models of HIV infection. J Transl Med. 2011 Jan 27;9 Suppl 1:S7. [view]
• Cheng-Mayer C, Huang Y, Gettie A, Tsai L, Ren W, Shakirzyanova M, Sina ST, Trunova N, Blanchard J, Jenkins LM, Lo Y, Schito ML, Appella E. Delay of simian human immunodeficiency virus infection and control of viral replication in vaccinated macaques challenged in the presence of a topical microbicide. AIDS. 2011 Sep 24;25(15):1833-1841. [view]
• Zhuang K, Finzi A, Tasca S, Shakirzyanova M, Knight H, Westmoreland S, Sodroski J, Cheng-Mayer C. Adoption of an "open" envelope conformation facilitating CD4 binding and structural remodeling precedes coreceptor switch in R5 SHIV-infected macaques. PLoS One. 2011;6(7):e21350. [view]
• Shakirzyanova M, Tsai L, Ren W, Gettie A, Blanchard J, Cheng-Mayer C. Pathogenic Consequences of Vaginal Infection with CCR5-Tropic Simian-Human Immunodeficiency Virus SHIVSF162P3N. J Virol. 2012;86(17):9432-42. [view]
• Mumbauer A, Gettie A, Blanchard J, Cheng-Mayer C. Efficient mucosal transmissibility but limited pathogenicity of R5 SHIVSF162P3N in Chinese origin rhesus macaques. J Acquir Immune Defic Syndr. 2012 Dec 6. [view]
• Zhuang K, Finzi A, Toma J, Frantzell A, Huang W, Sodroski J, Cheng-Mayer C. Identification of interdependent variables that influence coreceptor switch in R5 SHIV(SF162P3N)-infected macaques. Retrovirology. 2012 Dec 13;9:106. [view]
• Ren W, Mumbauer A, Zhuang K, Harbison C, Knight H, Westmoreland S, Gettie A, Blanchard J, Cheng-Mayer C. Mucosal transmissibility, disease induction and coreceptor switching of R5 SHIVSF162P3N molecular clones in rhesus macaques. Retrovirology. 2013 Jan 31;10(1):9. [view]

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Cecilia Cheng-Mayer	Professor
Rachel Ann Leda	Graduate Student
Wuze Ren	Post Doctoral Fellow
Madina Shakirzyanova	Post Doctoral Fellow
Li-Li Tsai	Research Technician
ZhengRong Yang	Visiting Scientist