Flow Cytometry

Becton Dickinson LSR II

The BD LSR II is an air-cooled 4-laser bench top flow cyto meter with the ability to acquire parameters for a 13 color experiment. It uses fixed-alignment lasers that transmit light reflected by mirrors through a flow cell to octagon and trigon detector arrays. These detectors collect and translate fluorescent signals into electronic signals. Instrument electronics convert these signals into digital data.

FACScalibur

The Flow Cytometry Facility at the Aaron Diamond AIDS Research Center supplies state-of-the-art capacity for cell analysis and sorting. The facility houses a Becton Dickinson FACScalibur 2-laser cell analyzer, capable of 4-color fluorescence data acquisition.

Analysis Workstation

There are also 2 advanced 8-core MacPros available for data analysis and a SGI octane workstation.

Software:

• Imaris "Surpass" Volume rendering in 3D and 4D, surface rendering, measurements, colocalization, slipping planes, 4D tracking
• SoftWoRx Deconvolution, volume rendering, surface rendering and quantification
• ModFit LT. This is a DNA analysis software featuring AutoDebris, AutoAggregates, AutoLinearity and batch processing
• FloJo Flow Cytometry Analysis software
• NIS Elements (Nikon) Image processing software

MoFlo Sorter

In addition, a Cytomation MoFlo high-performance cell sorter is available, configured with 4-lasers (one Coherent Argon/Krypton mixed gas, one Spectra-Physics Argon, one 405 nm 50 mW diode and one 635 nm 150 mW diode). We are currently capable of acquiring 8 colors of fluorescence. This system is capable of routine cell sorting at cell throughput rates of 50,000 cells per second. The MoFlo is contained in a BSL-3 facility, and is equipped with aerosol containment hardware for sorting infectious material.

The Biacore T200

The Biacore T200 is a versatile, label-free system for detailed studies of biomolecular interactions, from early research to drug discovery and development, and on to QC. The system delivers high quality kinetic, affinity, concentration, specificity, selectivity, and thermodynamic interaction data – in real time with exceptional sensitivity. Interactions characterized by on- and off-rates at the extremities of the kinetic scale can be analyzed.