DeltaVision Microscope
DeltaVision available optical filters
| Fluorochrome | Excitation (nm) | Emission (nm) |
| DAPI, Hoechst | 360/40 | 457/50 |
| Alexa Fluor 405 | 405/30 | |
| CFP | 436/10 | 470/30 |
| FITC, GFP | 490/20 | 538/38 |
| YFP | 500/20 | 535/30 |
| PE, Tx Red, Rhodamine, PKH26, Propidium Iodide |
555/28 | 617/73 |
| Alexa Fluor 568 | 575/25 | |
| Alexa Fluor 594, 7AAD, Nile Red | 630/40 | |
| APC, Cy5 | 640/20 | 685/40 |
DeltaVision objective lenses
| Lens ID# | Lens Type | Magnification/NA | Coupling Media | Working Distance (mm) | Depth of Field (um) | Comment |
| 10107 | UPlanApo | 10x/0.40 | Air | 3.1 | 3.16 | |
| 10205 | UApo/340 | 20x/0.75 | Air | 0.55 | 0.70 | |
| 10404 | UPlanApo | 40x/0.85 | Air | 0.20 | 0.56 | |
| 10403 | UApo/340 | 40x/1.35 | Oil | 0.10 | 0.32 | 0.65-1.35 NA |
| 10602 | PlanApo | 60x/1.40 | Oil | 0.10 | 0.28 | |
| 10603 | UPlanApo | 60x/1.20 | Water | 0.25 | 0.36 | |
| 10003 | UPlanApo | 100x/1.35 | Oil | 0.10 | 0.32 | 0.50-1.35 NA |
Image Gallery
Click to view a large version
GFP-Vpr labelled HIV-1 virus particles (Green) bound to the surface of target cells labeled with rhodamine-concanavalinA (red) and Hoechst 33258 (blue)
Image by Paul Bieniasz
and Theodora Hatziioannou
GFP-Vpr labelled HIV-1 virus particles (Green) bound to the surface of target cells labeled with rhodamine-concanavalinA (red) and Hoechst 33258 (blue) Image by Paul Bieniasz
and Theodora Hatziioannou
DeltaVision Primer
The following illustrates the technique of deconvolution and restoration microscopy as implemented on the API DeltaVision system.
This image was acquired from a DAPI-stained preparation of 293T cells, provided by Dr. Lubbertus Mulder at ADARC, acquired at 100X magnification.
The DeltaVision microscope is based upon a standard fluorescence microscope, with the addition of precision X, Y and Z stage control. After finding and focusing on a field of interest, the DeltaVision system is set up by the investigator to automatically acquire a number of images (image stack) while the system mechanically focuses through the cell at precise intervals. The following image is number 16 from an image stack of 20 images, each of which is an image taken as the focus was changed by 0.2 um.
This operation generates another image stack of deconvolved images, of which this is again the 16th image from the stack of 20. Notice the increase in resolution obtained by the removal of blurring.
This image stack may be further computer processed to yield a 3-D image of the cells.
Nikon Eclipse TS300 inverted microscope
Filter sets for GFP+DsRed or CFP+YFP, a Spot Insight QE color digital camera for brightfield and fluorescence imaging.
Filter sets for GFP+DsRed or CFP+YFP, a Spot Insight QE color digital camera for brightfield and fluorescence imaging.Other Equipment
Molecular Dynamics Store 840 Imaging Systems
The Store imaging system is an optical scanner that produces digital images of Labeled Samples. The Store 840 system includes the phosphor screen and the chemifluorescence/blue-excited fluorescence virtual instruments.
The Store imaging system is an optical scanner that produces digital images of Labeled Samples. The Store 840 system includes the phosphor screen and the chemifluorescence/blue-excited fluorescence virtual instruments. Applied Biosystems Real Time PCR
The Applied Biosystems Real Time PCR system (7500 system) uses fluorescent-based PCR chemistries to provide quantitative detection of nucleic acid sequence using real-time analysis and qualitative detection of nucleic acid sequence using end-point and dissociation curve analysis. The 7500 system allows you to perform several assay types with plates or tubes in the 96-well format.
The Applied Biosystems Real Time PCR system (7500 system) uses fluorescent-based PCR chemistries to provide quantitative detection of nucleic acid sequence using real-time analysis and qualitative detection of nucleic acid sequence using end-point and dissociation curve analysis. The 7500 system allows you to perform several assay types with plates or tubes in the 96-well format. Thermo Scientific NanoDrop1000
The Thermo Scientific NanoDrop1000 Spectrophotometer enables highly accurate UV/Vis analyses of 1 ul samples. The sample retention system eliminates the need for cuvettes and capillaries which decreases the measurement cycle time. In addition, the high absorbance capability eliminates the need for most dilutions.
The Thermo Scientific NanoDrop1000 Spectrophotometer enables highly accurate UV/Vis analyses of 1 ul samples. The sample retention system eliminates the need for cuvettes and capillaries which decreases the measurement cycle time. In addition, the high absorbance capability eliminates the need for most dilutions.