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The Flow Cytometry Facility at the Aaron Diamond AIDS Research Center supplies state of the art capacity for cell analysis and sorting. The facility houses a Becton Dickinson FACScalibur two laser cell analyzer, capable of 4 color fluorescence data acquisition. In addition, a Cytomation MoFlo high-performance cell sorter is available, configured with 3 lasers and currently capable of acquiring 8 colors of fluorescence. This system is capable of routine cell sorting at cell throughput rates of 50,000 cells per second. The MoFlo is contained in a BSL-3 facility, and is equipped with aerosol containment hardware for sorting infectious material.